Baseline examination (questionnaire and health examination). Questions were asked on social status and childhood, physical activity, smoking habits, hormonal status in women, body weight history, inflammatory disease in the past year, antibiotics and influenza vaccine, hypertension, diabetes and dyslipidemia and relative dietary and pharmacological treatment, ischemic cardiovascular disease and relative treatment and possible hospitalization, pulmonary diseases, depressive status or anxiety with relative previous and current treatment, haematological disease, kidney disease, gastrointestinal disease, endocrinological disease, Alzheimer disease, tumours, surgical intervention and radiological tests in the past five years. All medication in the past 15 days were recorded.

FFQ was used to determine daily nutritional intakes consumed in the past year.

Blood pressure and anthropometric measurements were taken.

Magnetic resonance imaging of brain was done for a sub-sample about 3,000 subjects. Electrocardiogram and spirometric tests were performed

Biological samples were taken and stored. Citrated plasma, EDTA plasma, serum, cellular pellet and urinary spots are stored in liquid nitrogen in a specific Biobank Biomarkers have been measured in the BiomarCaRE Project.

Re-examination of the cohort (questionnaire and health examination). The same assessments are done as in the baseline examination.

On top of the same examinations as in the baseline, also MoCa test, smell, hearing, taste and frailty assessment in over 65 years people were paerformed. Sleep quality was asked in the questionnaire, and bone density was examined.

This re-examination data is not harmonized in the MORGAM Project.

Deaths (all-cause and specific-cause) and hospitalizations (all-cause and specific-cause) are followed locally up to the year 2018.

Follow-up for cardiovascular events (includin heart failure and atrial fibrillation), type 2 diabetes, cancers, and death. See the end-points followed up in [a MORGAM e-publication](https://www.thl.fi/publications/morgam/cohorts/full/italy/ita-mola.htm).